Human LF / LTF / Lactoferrin

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-ENYKSQQSSDPDP, from the internal region of the protein sequence according to NP_002334.2.

aa433-445

Human LTF / Lactoferrin.

• IHC
• IHC - Paraffin (2 µg/ml)
• Western blot (0.05 - 0.2 µg/ml)
• ELISA (5 - 10 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:32000)

Immunohistochemistry: LS-B3025 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3025 was determined to be 2 ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 85kD band observed in Human Peripheral Mononucleocyte lysates (calculated MW of 78.2kD according to NP_002334.2). Recommended concentration: 0.05-0.2 ug/ml.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02788 NCBI: NM_002343 NP_002334.2