Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone A70-C/C8] (IgM) to Human Lewis y / CD174
IHC - Paraffin, IHC - Frozen
Human Lewis y / CD174
Human (tested or 100% immunogen sequence identity)
IgM Monoclonal [A70-C/C8]
IHC - Paraffin
IHC - Frozen
Specificity and Use
Lewis y / CD174 antibody was raised against live cells of the human colorectal carcinoma cell line Ls174T. The isolated splenocytes of the mouse were fused with the myeloma cell line X63-Ag8.653.
A70-C/C8 recognize the epitope Fuc alpha 1-2Gal(beta 1-4(Fuc alpha 1-3)GlcNAc beta 1 and is therefore specific for blood group antigen LewisY. The mAb has no cross-reactivity with the blood group H type 2 antigens or with Lea, sialyl-Lea, Lex, sialyl-Lex, Leb or Lec.