• Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA and Assay Kits
  • All Kits
  • Assay Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Immunohistochemistry Reagents
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Reviews
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Anti-Leu-Enkephalin Antibody (N-Terminus) LS-C23076

Catalog Size Price
LS-C23076-50 50 µg (1 mg/ml) Unavailable

Related Products

16 Leu-Enkephalin Antibodies

Most Popular Leu-Enkephalin Antibodies

Anti-Leu-Enkephalin Antibody (N-Terminus) LS-C23076
Rabbit Polyclonal (IgG) to Human Leu-Enkephalin
Human, Mouse, Rat
IHC - Frozen, ICC, Immunofluorescence
Anti-Leu-Enkephalin Antibody LS-C65346
Rabbit Polyclonal
IHC, ELISA, Radioimmunoassay
Anti-Leu-Enkephalin Antibody LS-C123457
Mouse Monoclonal (IgG)
IHC - Frozen, Radioimmunoassay
Anti-Leu-Enkephalin Antibody LS-C183848
Rabbit Polyclonal (IgG)
Anti-Leu-Enkephalin Antibody LS-C193696
Rabbit Polyclonal to Human Leu-Enkephalin
Human, Rat, Cat, Pig

100% Guaranteed
Rabbit Polyclonal (IgG) to Human Leu-Enkephalin
Human, Mouse, Rat
IHC - Frozen, ICC, Immunofluorescence


Human Leu-Enkephalin
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal


  • IHC - Frozen
  • ICC
  • Immunofluorescence

  • IHC - Paraffin

Specificity and Use

Leu-Enkephalin antibody was raised against synthetic Leu5-enkephalin peptide (H-Try-Gly-Gly-Phe-Leu-OH) conjugated through the N-terminal tyrosine to BSA.
Leu5-enkephalin. Cross-reactivity: Met5-enkephalin 0.93%, beta-endorphin 0.01% and beta-Lipotropin 0.01% Species Reactivity: Human, rat and mouse.
Immunohistochemistry: frozen sections 1:500-1:1,000 using immunofluorescence and 1:1,000-1:5,000 for indirect avidin-biotin. See protocol below. Immunohistochemistry Protocol:Anesthetize animals and perfuse them transcardially as follows:a) Flush with cold (4°C) oxygenated Calcium free Tyrodesb) Perfuse with 4% formaldehyde in 0.16M phosphate buffer at pH 6.9c) Perfuse with 10% sucrose solution in 0.1M phosphate buffer (pH 7.2)Sections: Cut 5-30um sections using a cryostat and mount them on subbed histological slides. Immunofluorescence:1. Dilute LS-C23076 with 0.1M PBS, pH 7.4, 1% BSA, 0.1% Triton X-100. 2. Incubate sections for 24-48 hours at 4°C.3. Wash in PBS (three times for 10 minutes). 4. Incubate for 1 hours at RT with properly diluted donkey anti-rabbit secondary antibody conjugated to a fluorescent probe such as FITC, Rhodamine or Cy3.5. Wash in PBS (three times for 10 minutes) and mount with a PBS/glycerol solution, 0.1% phenylenediamine to reduce fading. If not stained with FITC, sections can be dehydrated in grading alcohols (50%, 75%, 80%, 96% and 100%), cleared with xylene and mounted with DPX (Fluka, Ronkonkoma, NY). Staining can be visualized by using both conventional and confocal microscopy.Indirect Avidin-Biotin Peroxidase: 1. Incubate sections with 0.3% H2O2 in PBS for 15 minutes at RT to block endogenous peroxidase. 2. Rinse sections with PBS three times for 10 minutes. 3. Dilute LS-C23076 with 0.1M PBS, pH 7.4, 1% BSA, 0.1% Triton X-100. 4. Incubate sections overnight at 4°C and then wash in PBS (three times for 10 minutes). 5. Incubate sections with properly diluted biotinylated goat anti-rabbit secondary antibody diluted in PBS (do not add sodium azide!) for one hour at RT. 6. Rinse sections three times for 15 minutes and incubate sections with horseradish peroxidase-streptavidin complex properly diluted in PBS/0.1% Triton X-100 for 40 minutes at RT. 7. Wash sections with PBS and incubate them in substrate solutions (e.g. DAB, AEC, etc.) to achieve necessary intensity of ANP staining.


0.1% sodium azide as a preservative.
For research use only.

About Leu-Enkephalin

Requested From: 
Date Requested: 3/19/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy

Catalog Number