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Anti-Leptin Antibody (C-Terminus) IHC-plus™ LS-B4652

Note: This antibody replaces LS-C110649

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Rabbit Polyclonal to Human Leptin
Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Hamster, Horse, Sheep, Chicken
IHC - Paraffin, Western blot
Unconjugated

Details

Human Leptin
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Hamster, Horse, Sheep, Chicken (tested or 100% immunogen sequence identity)
Pig, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (0.2 - 1 µg/ml)

Specificity and Use

Leptin antibody was raised against synthetic peptide from C-Terminus of human LEP (P41159, NP_000221). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Goat, Hamster, Elephant, Zebu, Bovine, Horse, Turkey, Chicken (100%); Orangutan, Cat, Rabbit, Pig (92%); Panda (85%); Marmoset (84%).
LS-E15344 - Lyophilized - 100 µg - $145.00
C-Terminus
Human LEP / Leptin
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Leptin

P41159 NM_000230 NP_000221.1

LEP Antibody, Leptin Antibody, LEPD Antibody, Obese protein Antibody, Obese, mouse, homolog of Antibody, Obesity factor Antibody, OBS Antibody, OB Antibody

Leptin has been implicated in the control of appetite and metabolism.

Immunohistochemistry

Immunohistochemistry
Anti-LEP / Leptin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4652 concentration 5 ug/ml.

Western blot

Western blot
LEP / Leptin antibody LS-B4652 Western blot of HepG2 cell lysate.

Requested From: United States
Date Requested: 4/17/2015

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