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Anti-Leptin Antibody IHC-plus™ LS-B8192

Note: This antibody replaces LS-C104300, LS-C54171, LS-C89454, LS-C104734, LS-C25159

Ordering

Wt. Vol. Conc. Price
500 µg 500 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Leptin Antibodies

Anti-Leptin Antibody (N-Terminus) IHC-plus™ LS-B4651
Rabbit Polyclonal (IgG) to Human Leptin
Human, Mouse, Bovine, Dog, Hamster, Horse, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-Leptin Antibody (C-Terminus) IHC-plus™ LS-B4652
Rabbit Polyclonal to Human Leptin
Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Hamster, Horse, Sheep, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1,k) to Human Leptin
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human Leptin
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 0.4 µg/ml)
  • ELISA (2 - 4 µg/ml)

Specificity and Use

Leptin antibody was raised against e.coli derived recombinant Human Leptin.
Human Leptin
Immunohistochemistry: LS-B8192 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect recombinant human Leptin when used with biotinylated antigen affinity purified anti-human Leptin as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 2-4 ng/lane, under non-reducing conditions.

Packaging

Lyophilized from PBS
500 µl Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About Leptin

P41159 NM_000230 NP_000221.1

LEP Antibody, Leptin Antibody, LEPD Antibody, Obese protein Antibody, Obese, mouse, homolog of Antibody, Obesity factor Antibody, OBS Antibody, OB Antibody

Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing.

Immunohistochemistry

Immunohistochemistry
Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)
 

Requested From: United States
Date Requested: 12/4/2016

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