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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Leptin Antibody (aa134‑166) LS‑C100397
Leptin antibody LS-C100397 is an unconjugated rabbit polyclonal antibody to human Leptin. Validated for IF and WB.
Catalog
Size
Price
LS-C100397-400
400 µl
Unavailable

Popular Leptin Products

Anti-LEP / Leptin antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1459 concentration 5 ug/ml.
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-LEP / Leptin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4651 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Bovine, Dog, Hamster, Horse, Rabbit
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Anti-LEP / Leptin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4652 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Hamster, Horse, Sheep, Chicken
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Human, Kidney:Formalin-Fixed Paraffin-Embedded (FFPE)
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Applications: IHC, IHC - Paraffin, Western blot, ELISA
Detection limit for recombinant GST tagged LEP is approximately 0.1 ng/ml as a capture antibody.
Species: Human
Applications: Western blot, ELISA

Product Description

Leptin antibody LS-C100397 is an unconjugated rabbit polyclonal antibody to human Leptin. Validated for IF and WB.
About Leptin
Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. P41159 NM_000230 NP_000221.1

LEP Antibody, Leptin Antibody, LEPD Antibody, Obese protein Antibody, Obese, mouse, homolog of Antibody, Obesity factor Antibody, OBS Antibody, OB Antibody


Specifications

Target
Human Leptin
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Blocking Peptide
LS-E8097 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C100397. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa134-166
Specificity
This Leptin (LEP) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-166 amino acids from the C-terminal region of human Leptin (LEP).
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Confocal immunofluorescence of Leptin (LEP) Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of Leptin (LEP) Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of hLep-R149 in MDA-MB231 cell line lysates (35 ug/lane). LEP (arrow) was detected using the purified antibody.
Western blot of hLep-R149 in MDA-MB231 cell line lysates (35 ug/lane). LEP (arrow) was detected using the purified antibody.

Immunofluorescence

Confocal immunofluorescence of Leptin (LEP) Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of Leptin (LEP) Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of hLep-R149 in MDA-MB231 cell line lysates (35 ug/lane). LEP (arrow) was detected using the purified antibody.
Western blot of hLep-R149 in MDA-MB231 cell line lysates (35 ug/lane). LEP (arrow) was detected using the purified antibody.

Immunofluorescence

Confocal immunofluorescence of Leptin (LEP) Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of Leptin (LEP) Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of hLep-R149 in MDA-MB231 cell line lysates (35 ug/lane). LEP (arrow) was detected using the purified antibody.
Western blot of hLep-R149 in MDA-MB231 cell line lysates (35 ug/lane). LEP (arrow) was detected using the purified antibody.

Immunofluorescence

Confocal immunofluorescence of Leptin (LEP) Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of Leptin (LEP) Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of hLep-R149 in MDA-MB231 cell line lysates (35 ug/lane). LEP (arrow) was detected using the purified antibody.
Western blot of hLep-R149 in MDA-MB231 cell line lysates (35 ug/lane). LEP (arrow) was detected using the purified antibody.


Requested From: 
Date Requested: 6/25/2018

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