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Anti-LEMD3 / MAN1 Antibody (clone 6C10) LS-C114869


Wt. Vol. Conc. Price
- 100 µl 0.56 mg/ml $325

LSBio (Direct) LSBio (Direct)

Other LEMD3 / MAN1 Products

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Mouse Monoclonal [clone 6C10] (IgG1) to Human LEMD3 / MAN1
Human, Monkey, Dog
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry


Human LEMD3 / MAN1
Human, Monkey, Dog (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [6C10]
Protein A/G purified


IHC - Paraffin (1:50), Immunofluorescence (1:100), Western blot (1:1000), Flow Cytometry (1:100)

Specificity and Use

LEMD3 / MAN1 antibody was raised against full length human recombinant protein of human LEMD3 (NP_055134) produced in HEK293T cell.
Human LEMD3 / MAN1
Concentration: 0.5-1.0 mg/ml (Lot dependent)


PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About LEMD3 / MAN1

Q9Y2U8 NM_014319 NP_055134.2

LEMD3 Antibody, MAN1 Antibody, LEM domain containing 3 Antibody

Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest.


IHC of paraffin-embedded liver tissue using anti-LEMD3 mouse monoclonal antibody. (Dilution 1:50).


IHC of paraffin-embedded Carcinoma of liver tissue using anti-LEMD3 mouse monoclonal antibody. (Dilution 1:50).


Anti-LEMD3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LEMD3.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEMD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEMD3.

Western blot

Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-LEMD3 monoclonal antibody.

Flow Cytometry

Flow Cytometry
Flow cytometry of Jurkat cells, using anti-LEMD3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow Cytometry
Flow cytometry of HeLa cells, using anti-LEMD3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Requested From: United States
Date Requested: 11/26/2015

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