Human, Monkey, Bovine, Dog, Guinea pig
(tested or 100% immunogen sequence identity)
Pig, Rabbit (at least 90% immunogen sequence identity)
Also available conjugated with Biotin
- IHC - Paraffin (5 µg/ml)
- Western blot (0.1 - 2 µg/ml)
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
LEF1 antibody was raised against synthetic peptide located between aa51-100 of human LEF1 (Q9UJU2, NP_057353). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bovine (100%); Galago, Rabbit, Pig, Opossum (92%); Guinea pig (85%); Mouse, Rat (78%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B984. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunohistochemistry: LS-B984 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B984 was determined to be 5 ug/ml.
Lyophilized from PBS with 2% sucrose
Reconstitute with 50ul sterile ddH2O.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.