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Anti-LEF1 Antibody (aa51-100) IHC-plus™ LS-B984

Note: This antibody replaces LS-C29715
Catalog Size Price
LS-B984-50 50 µg (1 mg/ml) Unavailable

Related Products

58 LEF1 Antibodies

Most Popular LEF1 Antibodies

Anti-LEF1 Antibody (aa282-331) LS-C30951
Rabbit Polyclonal (IgG) to Human LEF1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-LEF1 Antibody (aa51-100) IHC-plus™ LS-B984
Rabbit Polyclonal to Human LEF1
Human, Monkey, Bovine, Dog, Guinea pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-LEF1 Antibody (Pro82) LS-C37241
Rabbit Monoclonal (IgG) to Human LEF1
Human, Mouse, Rat
ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Anti-LEF1 Antibody (aa10-37, FITC) LS-C237094
Rabbit Polyclonal (IgG) to Human LEF1
Human
Western blot, ELISA
FITC Conjugated
Anti-LEF1 Antibody LS-C499233
Rabbit Polyclonal (IgG) to Human LEF1
Human, Mouse
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Rabbit Polyclonal to Human LEF1
Human, Monkey, Bovine, Dog, Guinea pig
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human LEF1
Rabbit
Human, Monkey, Bovine, Dog, Guinea pig (tested or 100% immunogen sequence identity)
Pig, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 2 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

LEF1 antibody was raised against synthetic peptide located between aa51-100 of human LEF1 (Q9UJU2, NP_057353). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bovine (100%); Galago, Rabbit, Pig, Opossum (92%); Guinea pig (85%); Mouse, Rat (78%).
LS-E11347 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B984. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa51-100
Human LEF1
Immunohistochemistry: LS-B984 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B984 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
Reconstitute with 50ul sterile ddH2O.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About LEF1

Q9UJU2 NM_016269 NP_057353.1

LEF1 Antibody, LEF-1 Antibody, TCF7L3 Antibody, TCF1-alpha Antibody, TCF10 Antibody, TCF1ALPHA Antibody

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity).

Immunohistochemistry

Immunohistochemistry
Anti-LEF1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B984 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-LEF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B984 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
LEF1 antibody LS-B984 Western blot of 293T Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 10/22/2017

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