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Anti-LEF1 Antibody (aa257-270) LS-C34665

Note: This antibody replaces LS-C71859, LS-C71854

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular LEF1 Antibodies

Anti-LEF1 Antibody (aa282-331) LS-C30951
Rabbit Polyclonal (IgG) to Human LEF1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human LEF1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Chicken, Zebrafish
Western blot, ELISA
Unconjugated

Details

Human LEF1
Goat
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1 - 3 µg/ml)
  • ELISA (1:32000)

  • IHC - Paraffin

Specificity and Use

LEF1 antibody was raised against synthetic peptide C-QHEQRKEQEPKRPH from an internal region of human LEF1 (NP_057353.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Stickleback, Zebrafish (100%); Xenopus (93%); Pufferfish (86%).
LS-E26071 - Lyophilized - 100 µg - $145.00
aa257-270
Human LEF1.
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2 kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3;21(11):2703-14.; PMID: 12032083). Recommended concentration: 1-3 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About LEF1

Q9UJU2 NM_016269 NP_057353.1

LEF1 Antibody, LEF-1 Antibody, TCF7L3 Antibody, TCF1-alpha Antibody, TCF10 Antibody, TCF1ALPHA Antibody

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity).

Western blot

Western blot
LEF1 antibody (1 ug/ml) staining of Daudi cell lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/11/2016

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