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Anti-LDHA / LDH1 Antibody (clone 2D11) LS-C114665

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.75 mg/ml $325
Inquire for larger quantities

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206-374-1102
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Most Popular LDHA / LDH1 Antibodies

Anti-LDHA / LDH1 Antibody (clone 2D11) LS-C114665
Mouse Monoclonal [clone 2D11] (IgG1) to Human LDHA / LDH1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-LDHA / LDH1 Antibody (aa280-332) LS-C143436
Rabbit Polyclonal to Human LDHA / LDH1
Human, Bovine
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-LDHA / LDH1 Antibody IHC-plus™ LS-B6423
Mouse Monoclonal (IgG1) to Human LDHA / LDH1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2D11] (IgG1) to Human LDHA / LDH1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human LDHA / LDH1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2D11]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Western blot (1:1000)
  • Immunoprecipitation

Specificity and Use

LDHA / LDH1 antibody was raised against full length human recombinant protein of human LDHA (NP_005557) produced in HEK293T cell.
Human LDHA / Lactate Dehydrogenase A
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About LDHA / LDH1

P00338 NM_005566 NP_005557.1

LDHA Antibody, LDH muscle subunit Antibody, LDH1 Antibody, Lactate dehydrogenase M Antibody, LDH-M Antibody, GSD11 Antibody, Lactate dehydrogenase A Antibody, LDH-A Antibody, LDHM Antibody, PIG19 Antibody, Proliferation-inducing gene 19 Antibody

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Carcinoma of bladder tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LDHA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LDHA.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation(IP) of LDHA by using monoclonal anti-LDHA antibodies (Negative control: IP without adding anti-LDHA antibody.). For each experiment, 500ul of DDK tagged LDHA overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-LDHA antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Requested From: United States
Date Requested: 12/9/2016

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