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Anti-LDH / Lactate Dehydrogenase Antibody LS-C59234

Note: This antibody replaces LS-C25046

Ordering

Wt. Vol. Conc. Price
- 2000 µl - $1,110
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular LDH / Lactate Dehydrogenase Antibodies

Anti-LDH / Lactate Dehydrogenase Antibody (HRP) LS-C209195
Goat Polyclonal to Rabbit LDH / Lactate Dehydrogenase
Rabbit
IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
HRP Conjugated
Immunofluorescence Image
Anti-LDH / Lactate Dehydrogenase Antibody LS-C147498
Rabbit Polyclonal to Bovine LDH / Lactate Dehydrogenase
Bovine
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Rabbit LDH / Lactate Dehydrogenase
Rabbit
ICC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Rabbit LDH / Lactate Dehydrogenase
Goat
Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • ICC
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:4000 - 1:20000)

Specificity and Use

Full length lactate dehydrogenase protein isolated from rabbit muscle.
Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species
This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 36 kD in size corresponding to LDH western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
2,000 µl deionized water
Store at 4°C.
For research use only.

About LDH / Lactate Dehydrogenase

Lactate dehydrogenase is composed of four subunits (tetramer). The two most common subunits are the LDH-M and LDH-H protein, encoded by the LDHA and LDHB genes, respectively. These two subunits can form five possible tetramers (isoenzymes): 4H, 4M, and the three mixed tetramers (3H1M, 2H2M, 1H3M).

Immunofluorescence

Immunofluorescence
Lactate Dehydrogenase Antibody-Immunofluorescence. Biotin conjugated anti-lactate dehydrogenase antibody was used to stain HeLa cells by immunofluorescence. HeLa cells were plated in 12 well plates, fixed for 5 min in 1:1 Methanol: Acetone, blocked with MB-071 (preservative free) for 15 min and stained 1 hr with antibody 1:200 in blocking buffer. Plate was washed 3X in PBS, and primary biotin conjugate was detected by DyLight 488 conjugated Streptavidin (S000-41 lot 21097) 1:10000 for 30 min. Well was washed 3X in PBS.

Western blot

Western blot
Anti-Lactate Dehydrogenase Antibody - Western Blot. Western blot analysis shows Anti-Lactate Dehydrogenase antibody detects LDH in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). ~30 ug was loaded per lane onto a 10% gel for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kD corresponding to the expected molecular weight for the protein. The blot was incubated with a 1:1000 dilution of the antibody in 5% milk in TBST at room temperature followed by detection using standard procedures. Personal communication Kuldeep Patel.

Requested From: United States
Date Requested: 12/10/2016

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