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Anti-LDB1 / CLIM2 Antibody (aa86-411) LS-C185626


Wt. Vol. Conc. Price
- 100 µl 0.93 mg/ml Unavailable

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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human LDB1 / CLIM2
Human, Mouse
IHC - Paraffin, ICC, Western blot, Immunoprecipitation


Human LDB1 / CLIM2
Human, Mouse (tested or 100% immunogen sequence identity)
Rat, Chicken, Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
  • Immunoprecipitation

Specificity and Use

LDB1 / CLIM2 antibody was raised against recombinant fragment corresponding to a region within amino acids 86 and 411 of LDB1 (SwissProt Q86U70). Percent identity by BLAST analysis: Human (100%); Rat (99%); Chicken, Xenopus (97%).
Human CLIM2 / LDB1
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).


0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About LDB1 / CLIM2

Q86U70 NM_003893 NP_003884.1

LDB1 Antibody, CLIM-2 Antibody, HLdb1 Antibody, LIM domain-binding factor-1 Antibody, LIM domain-binding protein 1 Antibody, NLI Antibody, LIM domain binding 1 Antibody, CLIM2 Antibody, LDB-1 Antibody, Nuclear LIM interactor Antibody

Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression.


IHC of paraffin-embedded U87 Xenograft using LDB1 antibody at 1:100 dilution.


Immunofluorescence of paraformaldehyde-fixed HeLa using LDB1 antibody at 1:200 dilution.

Western blot

Western blot
Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. CLIM2 / LDB1 antibody diluted at 1:1000.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: Raji. 10% SDS PAGE. CLIM2 / LDB1 antibody diluted at 1:1000.


LDB1antibody immunoprecipitates LDB1 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract. A. 20 ug 293T whole cell lysate/extract. B. Control with 2.5 ug of preimmune rabbit IgG. C. Immunoprecipitation of LDB1 protein by 2.5 ug of LDB1 antibody . 12% SDS-PAGE. The immunoprecipitated LDB1 protein was detected by LDB1antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Requested From: 
Date Requested: 4/22/2017

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