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| Catalog Number | Size | Price |
|---|---|---|
| LS-B983-100 | 100 µl | $485 |
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Polyclonal Rabbit anti‑Human LDB1 / CLIM2 Antibody (aa303‑352, IHC, WB) LS‑B983
Polyclonal Rabbit anti‑Human LDB1 / CLIM2 Antibody (aa303‑352, IHC, WB) LS‑B983
Note: This antibody replaces LS-C30448
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
CLIM2 antibody LS-B983 is an unconjugated rabbit polyclonal antibody to human CLIM2 (LDB1) (aa303-352). Validated for IHC and WB.
Target
Human LDB1 / CLIM2
Synonyms
LDB1 | CLIM-2 | HLdb1 | LIM domain-binding factor-1 | LIM domain-binding protein 1 | NLI | LIM domain binding 1 | CLIM2 | LDB-1 | Nuclear LIM interactor
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide located between aa303-352 of human LDB1 (Q86U70-2, NP_003884). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Xenopus, Zebrafish (100%); Platypus (92%).
Epitope
aa303-352
Specificity
Human LDB1
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.2 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B983 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B983 was determined to be 5 ug/ml.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About LDB1 / CLIM2
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Featured Products
![LDB1 / CLIM2 Antibody - Anti-LDB1 Antibody - Western Blot. Western blot of affinity purified anti-LDB1 antibody shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 ug of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1800 dilution in 5% BLOTTO in PBS reacted overnight at 4C. The membrane was washed and reacted with a 1:20000 dilution of DyLight800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com//image2/ldb1-clim2-antibody-c-terminus-ls-c19038/62806_879894.jpg)
Species:
Mouse, Human, Rat
Applications:
Western blot, ELISA, Chromatin Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Reactivity:
Monkey
Range:
15.63-1000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
