Monoclonal Mouse anti‑Human LCP2 / SLP‑76 Antibody (clone SLP‑76/03, Concentrated, aa216‑434, WB) LS‑C204152
Monoclonal Mouse anti‑Human LCP2 / SLP‑76 Antibody (clone SLP‑76/03, Concentrated, aa216‑434, WB) LS‑C204152
Antibody:
LCP2 / SLP-76 Mouse anti-Human Monoclonal (aa216-434) (Concentrated) (SLP-76/03) Antibody
Format:
Unconjugated, Concentrated
Overview
Antibody:
LCP2 / SLP-76 Mouse anti-Human Monoclonal (aa216-434) (Concentrated) (SLP-76/03) Antibody
Format:
Unconjugated, Concentrated
Specifications
Description
SLP-76 antibody LS-C204152 is an unconjugated mouse monoclonal antibody to human SLP-76 (LCP2) (aa216-434). Validated for WB.
Target
Human LCP2 / SLP-76
Synonyms
LCP2 | 76 kDa tyrosine phosphoprotein | Lymphocyte cytosolic protein 2 | SLP-76 | SLP-76 tyrosine phosphoprotein | SLP76
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Modifications
Concentrated
Immunogen
A fusion protein representing amino acids 216-434 of human SLP-76
Specificity
SLP-76 (SH2 domain-containing leukocyte protein) is an adaptor molecule that is involved in T cell development and activation. Thymocytes from transgenic mice expressing a SLP-76 mutant with deletion of the amino terminus are unable to develop to CD4-CD8- stage. Mature T cells do develop in mice expressing SLP-76 with mutations in proline rich region or SH2 domain, but they fail to upregulate CD69 or proliferate appropriately in response to anti-CD3 stimulation. SLP-76 is cytosolic protein identified as phosphoprotein migrating at 76 kD in SDS-PAGE. Amino-terminal domain of SLP-76 containing several tyrosines is followed by PR (proline rich) region and an SH2 domain. SLP-76 associates with LAT via Gads or Grb 2. The PR region of SLP-76 constitutively associates with carboxy-terminal SH3 domain of Gads and P-1 segment of the PR region was also identified that associates with the SH3 domain of PLC-gamma1. The PR region therefore provides another pathway for PLC-gamma1 recruitment to the LAT signalling complex in T cells. The SH2 domain of SLP-76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human
Applications:
Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Source:
E. coli
Tag:
T7, N-terminus + 6His, C-terminus
Source:
E. coli
Tag:
His, N-Terminal
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024