Human, Monkey, Rat, Bat, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Mouse, Bovine, Guinea pig (at least 90% immunogen sequence identity)
Western blot (0.2 - 1 µg/ml)
Specificity and Use
LCA3 / SPATA7 antibody was raised against synthetic peptide located between aa252-301 of human SPATA7 (Q9P0W8, NP_060888). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Bat, Horse, Pig, Opossum (100%); Mouse, Bovine, Guinea pig (92%); Elephant (90%); Rat, Dog, Platypus (85%).
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Lyophilized from PBS with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.