Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IHC - Paraffin
Specificity and Use
LAX1 / LAX antibody was raised against synthetic peptide C-PLLTLPQTRQRA from an internal region of human LAX1 (NP_060243.2; NP_001129662.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%); Monkey (92%); Elephant, Bovine, Bat, Pig (83%).
LS-E25596 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C87199. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human LAX1. This antibody is expected to recognise both reported isoforms.
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave bands at approx. 100kD and 26kD in Human Tonsil lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 44.1kD according to NP_060243.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?