Human Laminin

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity chromatography

Unmodified

Anti-Laminin Antibody was produced by repeated immunizations with a mixture of synthetic peptides corresponding to regions of adherence on laminin.

Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Anti-Laminin (Human) (RABBIT) Antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1:500 - 1:,000)
• Immunoprecipitation (1:100)
• ELISA (1:4000 - 1:8000)

Anti-Laminin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. This product was assayed by immunoblot and found to be reactive against Laminin at a dilution of 1:5000 to 1:10000. This product was also assayed against 1.0 ug of Laminin in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884 and ABTS as a substrate for 30 minutes at room temperature.

0.125 M Sodium Borate, pH 8.0, 0.005 M EDTA, 0.075 M NaCl, 0.01% Sodium Azide

Store at 4°C.

For research use only. Intended for use by laboratory professionals.

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