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Anti-Laminin Antibody (Biotin) LS-C60152

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Laminin Antibodies

Anti-Laminin Antibody LS-C25112
Mouse Monoclonal (IgG1) to Human Laminin
Human
IHC - Paraffin, IHC - Frozen, ICC, Western blot, ELISA
Unconjugated
Anti-Laminin Antibody LS-C96142
Chicken Polyclonal to Human Laminin
Human, Mouse, Rat
IHC, ELISA
Unconjugated
Anti-Laminin Antibody IHC-plus™ LS-B4754
Rabbit Polyclonal to Mouse Laminin
Mouse, Human, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human Laminin
Human
Western blot, Immunoprecipitation, ELISA
Biotin Conjugated

Details

Human Laminin
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Biotin
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:10000)
  • Immunoprecipitation
  • ELISA

Specificity and Use

Laminin antibody was raised against non-reduced 400 kD human laminin. Immunization occurred after purity assessment of native protein in non-reducing SDS-PAGE system.
other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Laminin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Nonspecific cross reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible
Anti-Laminin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. This product was assayed by immunoblot and found to be reactive against Laminin at a dilution of 1:5000 to 1:10000. This product was also assayed against 1.0 ug of Laminin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4000 to 1:8000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide.
Store at 4°C.
For research use only.

About Laminin

Laminins are high-molecular weight (~400kDa) proteins of the extracellular matrix. They are a major component of the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, and adhesion, as well as phenotype and survival.

Requested From: United States
Date Requested: 12/3/2016

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