Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal (IgG) to Mouse Laminin A / Laminin B
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Mouse Laminin A / Laminin B
Mouse (tested or 100% immunogen sequence identity)
IHC - Paraffin
IHC - Frozen
Specificity and Use
Laminin isolated from mouse EHS tumor
As original characterized, clone AL-4 reacts with EHS mouse laminin, A chain (laminin 1). The epitope is mapped to the carboxy terminal globular domain on the A chain. Recent work by Scheele et al (2006) has further demonstrated that clone AL-4, under reducing conditions, recognized both the 200 kD 1/1 band and the 400 kD 1 band of mouse EHS laminin. However the affinity for the 400 kD band was very low. AL-4 also demonstrated strong binding the Laminin 1. By ELISA the epitope for the AL-4 seemed to be located on the Laminin 1 chain, however the clone also recognizes the laminin 1 chain in immunoblots, thus AL-4 seems to show mixed cross reactivity to laminins 1 and 1, with the 1 reactivity being more prominent
ELISA/RIA. Western blot. Immunoprecipitation. Immunohistochemistry. Reactive on tissue frozen in OCT at -70°C. Sections may be treated with hyaluronidase. Shows reactivity to paraffin embedded EHS tumor.
0.02 M PB, pH 7.6, 0.25 M sodium chloride, 0.1% sodium azide