Human Lamin C

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Affinity purified

Unmodified

A peptide made from the last 8 amino acids of Lamin C, including an N-Terminus lysine as a linker (KHHVSGSRR), coupled to KLH

N-Terminus

This antibody reacts strongly in immunoblotting with fragments corresponding to peptide 319-572aa of Lamin C. When tested against nuclear protein extracts from HeLa cells it reacts with a single band migrating at 65 kD. Stains Lamin C from human, mouse and rat material

• IHC
• IHC - Paraffin (1:50)
• IHC - Frozen
• Immunofluorescence
• Western blot

Immunohistochemistry: LS-B2972 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2972 was determined to be 1:50.

PBS, 0.1% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.