Target
Human LAIR2 / CD306
Synonyms
LAIR2 | CD306 | CD306 antigen | LAIR-2
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NSO-derived rhLAIR2.
Specificity
Recognizes human LAIR2 in direct ELISAs and Western Blots. Does not rhLAIR1, rmLAIR1, or rmLAIR2.
Applications
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (25 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in Western Blot, Flow Cytometry and ELISA. Flow Cytometry: 25 ug/ml Add 10 ul to 1-5x10^5 cells in a total reaction volume not exceeding 200ul. For intracellular staining, cells must be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Western Blot:1-2 ug/ml Using a colorimetric detection system the detection limit is ~5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 ug/ml. The detection limit is ~5 ng/well.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee