(tested or 100% immunogen sequence identity)
Also available Unconjugated
Protein G purified
- Flow Cytometry (1:1 - 1:5)
- (applications tested for the base form of this product only)
LAG3 antibody was raised against murine CD223 Ig fusion protein.
Recognizes murine lymphocyte activation gene-3 (LAG-3), a 70kD activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Recognizes an epitope within the D2 domain of CD223. The antibody is reported to block the in vitro function of murine LAG-3 but studies suggest that the antibody does not block binding of LAG-3 to MHC class II. XXX recommend the use of MCA2386EL for this purpose.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Lyophilized powder from PBS, pH 7.4, 0.09% sodium azide, 1% BSA, 5% sucrose.
100 tst Reconstitute with 1.0 ml distilled water.
Store at 4°C. Do not freeze. Protect from light.
For research use only.
This antibody carries the LSBio 100% Guarantee.