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Kv10.1 / KCNH1

potassium voltage-gated channel, subfamily H (eag-related), member 1

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. It is activated at the onset of myoblast differentiation. The gene is highly expressed in brain and in myoblasts. Overexpression of the gene may confer a growth advantage to cancer cells and favor tumor cell proliferation. Alternative splicing of this gene results in two transcript variants encoding distinct isoforms.

Gene Name: potassium voltage-gated channel, subfamily H (eag-related), member 1
Family/Subfamily: Ion Channel , Potassium channel - Eag-related
Synonyms: KCNH1, EAG1, EAG, EAG channel 1, HEAG1, H-eag, Kv10.1
Target Sequences: NM_002238 NP_002229.1 O95259

Publications (2)

1
Purification of an EH domain-binding protein from rat brain that modulates the gating of the rat ether--go-go channel. Piros ET, Shen L, Huang XY. The Journal of biological chemistry. 1999 274:33677-83. [PubMed:10559257]
2
Human ether--go-go gene potassium channels are regulated by EGFR tyrosine kinase. Wu W, Dong MQ, Wu XG, Sun HY, Tse HF, Lau CP, Li GR. Biochimica et biophysica acta. 2012 1823:282-9. [PubMed:22061963]

☰ Filters
Antibodies (16)
Proteins (6)
Over-Expression Lysate (4)
Primary (16)
Recombinant (2)
Kv10.1 / KCNH1 (22)
Human (15)
Mouse (11)
Rat (11)
Bovine (1)
Zebrafish (1)
IHC (3)
IHC-P (2)
WB (14)
Flo (1)
ELISA (7)
IF (1)
Peptide-ELISA (3)
rabbit (16)
Ig (1)
IgG (11)
polyclonal pc (16)
Unconjugated (16)
690-770 aa, Internal (1)
C-Terminus (1)
Internal (1)
aa703-752 (1)
aa720-769 (1)
aa762-807 (1)
No (22)
ABP-His, N-terminus (1)
GST, N-terminus (1)
Myc-DDK (Flag) (4)
Human (4)
293T Cells (2)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Kv10.1 / KCNH1 Antibody - All lanes: Anti-KCNH1 Antibody (C-Term) at 1:2000 dilution Lane 1: COLO 205 whole cell lysate Lane 2: Human brain lysate Lane 3: Hela whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 111 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
ELISA, Flo, WB
Unconjugated
200 µl/$393
Kv10.1 / KCNH1 Antibody - Immunohistochemistry of paraffin-embedded human brain tissue at dilution 1:100
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µl/$294; 100 µl/$360
Kv10.1 / KCNH1 Antibody - Immunohistochemistry of paraffin-embedded human colon cancer at dilution 1:100
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µl/$294; 100 µl/$360
Kv10.1 / KCNH1 Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
Kv10.1 / KCNH1 Antibody - Western blot analysis of extracts of various cell lines.
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
Kv10.1 / KCNH1 Antibody - Western blot testing of 1) rat brain and 2) mouse brain lysate with KCNH1 antibody at 0.5ug/ml. Predicted molecular weight ~111 kDa (may be observed larger than predicted due to glycosylation).
Select
Kv10.1 / KCNH1 Rabbit anti-Mouse Polyclonal Antibody
Mouse, Rat
ELISA, WB
Unconjugated
100 µg/$575
Kv10.1 / KCNH1 Antibody - Western blot analysis of KCNH1 using anti-KCNH1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNH1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KCNH1 at approximately 160KD. The expected band size for KCNH1 is at 111KD.
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
10 µg/$318; 100 µg/$470
Kv10.1 / KCNH1 Antibody - Western blot of KCNH1 antibody
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal (690-770 aa, Internal) Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
Kv10.1 / KCNH1 Antibody - Western blot analysis of extracts from HuvEc cells, using KCNH1 antibody.
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal (aa720-769) Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
Kv10.1 / KCNH1 Antibody - Western blot analysis of the lysates from COLO205 cells using KCNH1 antibody.
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal (aa703-752) Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal (aa762-807) Antibody
Mouse, Rat, Human
WB
Unconjugated
50 µl/$321; 100 µl/$403
Kv10.1 / KCNH1 Antibody - Western blot - Anti-KCNH1 Picoband antibody
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
10 µg/$318; 100 µg/$470
Kv10.1 / KCNH1 Antibody - Western blot analysis of Kv10.1 expression in HeLa (A); NIH3T3 (B); H9C2 (C) whole cell lysates.
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal (Internal) Antibody
Zebrafish, Rat, Mouse, Bovine, Human
WB
Unconjugated
100 µl/$299
Kv10.1 / KCNH1 Antibody - Anti-Kv10.1 Antibody at 1:1000 dilution + HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 111 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
100 µl/$386
Kv10.1 / KCNH1 Antibody - Western blot testing of human 1) COLO-320, 2) HepG2 and 3) A549 lysate with KCNH1 antibody at 0.5ug/ml. Predicted molecular weight ~111 kDa (may be observed larger than predicted due to glycosylation).
Select
Kv10.1 / KCNH1 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
100 µg/$575
Kv10.1 / KCNH1 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Select
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
Kv10.1 / KCNH1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
111.2 kDa
20 µg/$215
Kv10.1 / KCNH1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
108.4 kDa
20 µg/$215
Kv10.1 / KCNH1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
111.2 kDa
100 µg/$710
Kv10.1 / KCNH1 Protein
Select
E. coli
ABP-His, N-terminus
26.9 kDa
50 µg/$419
Kv10.1 / KCNH1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
108.4 kDa
100 µg/$710
Viewing 1-22 of 22 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).