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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KRT8 / CK8 / Cytokeratin 8 Antibody (clone AT3D11) IHC‑plus™ LS‑B9664
Note: This antibody replaces LS-C200836
Cytokeratin 8 antibody LS-B9664 is an unconjugated mouse monoclonal antibody to human Cytokeratin 8 (KRT8 / CK8). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B9664-50
50 µl (1 mg/ml)
Unavailable

Popular KRT8 / CK8 / Cytokeratin 8 Products

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Formalin-fixed, paraffin-embedded human breast carcinoma stained with peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Product Description

Cytokeratin 8 antibody LS-B9664 is an unconjugated mouse monoclonal antibody to human Cytokeratin 8 (KRT8 / CK8). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About KRT8 / CK8 / Cytokeratin 8
KRT8 / CK8 / Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. P05787 NM_002273 NP_002264.1

KRT8 Antibody, CARD2 Antibody, CK-8 Antibody, CYK8 Antibody, Cytokeratin-8 Antibody, CK8 Antibody, K2C8 Antibody, K8 Antibody, KO Antibody, Keratin-8 Antibody, Type-II keratin Kb8 Antibody, Cytokeratin 8 Antibody, Keratin 8 Antibody


Specifications

Target
Human KRT8 / CK8 / Cytokeratin 8
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG3,k Monoclonal [AT3D11]
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human KRT8 (1-483aa) purified from E. coli.
Specificity
Human KRT8 / CK8 / Cytokeratin 8
Presentation
Supplied in PBS, pH 7.4, 10% glycerol, 0.02% sodium azide
Storage
Can be stored at 4°C. For long term storage, aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-KRT8 / CK8 / Cytokeratin 8 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9664 concentration 10 ug/ml.
Anti-KRT8 / CK8 / Cytokeratin 8 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9664 concentration 10 ug/ml.

Western blot

Western Blot: The cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: The cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Anti-KRT8 / CK8 / Cytokeratin 8 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9664 concentration 10 ug/ml.
Anti-KRT8 / CK8 / Cytokeratin 8 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9664 concentration 10 ug/ml.

Western blot

Western Blot: The cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: The cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Anti-KRT8 / CK8 / Cytokeratin 8 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9664 concentration 10 ug/ml.
Anti-KRT8 / CK8 / Cytokeratin 8 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9664 concentration 10 ug/ml.

Western blot

Western Blot: The cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: The cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Anti-KRT8 / CK8 / Cytokeratin 8 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9664 concentration 10 ug/ml.
Anti-KRT8 / CK8 / Cytokeratin 8 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9664 concentration 10 ug/ml.

Western blot

Western Blot: The cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: The cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.


Requested From: 
Date Requested: 6/23/2018

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