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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KRT72 / Keratin 72 / K6irs Antibody LS‑C155482 Best Seller
K6irs antibody LS-C155482 is an unconjugated rabbit polyclonal antibody to human K6irs (KRT72 / Keratin 72). Validated for IHC and WB.
Catalog
Size
Price
LS-C155482-100
100 µl (1 mg/ml)
Unavailable

Popular KRT72 / Keratin 72 / K6irs Products

Antibody
Species: Human, Mouse
Applications: IHC, Western blot
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

K6irs antibody LS-C155482 is an unconjugated rabbit polyclonal antibody to human K6irs (KRT72 / Keratin 72). Validated for IHC and WB.
About KRT72 / Keratin 72 / K6irs
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. The type II keratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. KRT72 / Keratin 72 / K6irs encodes a type II keratin that is specifically expressed in the inner root sheath of hair follicles. Q14CN4 NM_080747 NP_542785.1

KRT72 Antibody, CK-72 Antibody, K6irs Antibody, K72 Antibody, Keratin protein K6irs Antibody, K6IRS2 Antibody, KRT6IRS2 Antibody, KB35 Antibody, Keratin 72 Antibody, Type-II keratin Kb35 Antibody, Cytokeratin-72 Antibody, Keratin-72 Antibody, KRT6 Antibody


Specifications

Target
Human KRT72 / Keratin 72 / K6irs
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 217 and 434 of Cytokeratin 72. Percent identity by BLAST analysis: Human (100%); Bovine (89%).
Specificity
Human KRT6 / Cytokeratin 6
Usage
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Presentation
0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
Storage
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Colon ca, using KRT72 antibody at 1:500 dilution.
IHC of paraffin-embedded Colon ca, using KRT72 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE KRT6 / Cytokeratin 6 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE KRT6 / Cytokeratin 6 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Colon ca, using KRT72 antibody at 1:500 dilution.
IHC of paraffin-embedded Colon ca, using KRT72 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE KRT6 / Cytokeratin 6 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE KRT6 / Cytokeratin 6 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Colon ca, using KRT72 antibody at 1:500 dilution.
IHC of paraffin-embedded Colon ca, using KRT72 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE KRT6 / Cytokeratin 6 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE KRT6 / Cytokeratin 6 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded Colon ca, using KRT72 antibody at 1:500 dilution.
IHC of paraffin-embedded Colon ca, using KRT72 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE KRT6 / Cytokeratin 6 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE KRT6 / Cytokeratin 6 antibody diluted at 1:1000


Requested From: 
Date Requested: 6/22/2018

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