LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us

KRT6 / CK6 / Cytokeratin 6 Antibody (aa545-555, clone SPM269)

Cytokeratin 6 antibody LS-C392262 is an unconjugated mouse monoclonal antibody to Cytokeratin 6 (KRT6 / CK6) from human, horse and sheep. Validated for Flow, IF and IHC.
Catalog
Size
Price
LS-C392262-20
20 µg (0.2 mg/ml)
$255
LS-C392262-100
100 µg
$395
Description
Cytokeratin 6 antibody LS-C392262 is an unconjugated mouse monoclonal antibody to Cytokeratin 6 (KRT6 / CK6) from human, horse and sheep. Validated for Flow, IF and IHC.
Target
Human KRT6 / CK6 / Cytokeratin 6
Host
Mouse
Reactivity
Human, Horse, Sheep (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bat, Dog, Hamster (at least 90% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [SPM269]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Ready-to-Use, Azide-free.
Applications
  • IHC
  • IHC - Paraffin (0.1 - 0.2 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human keratin 6 (GSSTIKYTTTS, aa545-555 of UniProt P48668). Percent identity by BLAST analysis: Human, Sheep, Horse (100%); Mouse, Rat, Ferret, Cat, Bat, Hamster, Elephant, Dog (91%); Bovine, Pig, Opossum (82%).
Epitope
aa545-555
Specificity
Human KRT6 / CK6 / Cytokeratin 6
Usage
In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation. The optimal dilution of the Cytokeratin 6 antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Presentation
0.01% BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.

Popular KRT6 / CK6 / Cytokeratin 6 Products

Antibody
Species: Human, Horse
Applications: IHC
Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
Antibody
Species: Human, Chimpanzee, Orangutan, Gibbon, Horse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
IHC testing of FFPE human bladder carcinoma and Cytokeratin 6 antibody. This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon, Horse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA

Publications (0)


Customer Reviews (0)


Images

Immunohistochemistry

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 6 antibody (SPM269).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 6 antibody (SPM269). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 6 antibody (SPM269).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 6 antibody (SPM269). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 6 antibody (SPM269).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 6 antibody (SPM269). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 6 antibody (SPM269).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 6 antibody (SPM269). This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 1/23/2019
Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2019 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy