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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KRT5 / CK5 / Cytokeratin 5 Antibody (clone 4A7) LS‑C175541
Cytokeratin 5 antibody LS-C175541 is an unconjugated mouse monoclonal antibody to human Cytokeratin 5 (KRT5 / CK5). Validated for IHC and WB.
Catalog
Size
Price
LS-C175541-100
100 µl (1 mg/ml)
Unavailable

Popular KRT5 / CK5 / Cytokeratin 5 Products

Antibody
Species: Human, Mouse, Bovine
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry
Anti-KRT5 / Cytokeratin 5 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3359 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin
Anti-KRT5 / Cytokeratin 5 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3463 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-KRT5 / Cytokeratin 5 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5496 dilution 1:200.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-KRT5 / CK5 / Cytokeratin 5 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

Cytokeratin 5 antibody LS-C175541 is an unconjugated mouse monoclonal antibody to human Cytokeratin 5 (KRT5 / CK5). Validated for IHC and WB.
About KRT5 / CK5 / Cytokeratin 5
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. P13647 NM_000424 NP_000415.2

KRT5 Antibody, 58 kd cytokeratin Antibody, 58 kDa cytokeratin Antibody, CK5 Antibody, CK-5 Antibody, Cytokeratin-5 Antibody, DDD Antibody, EBS2 Antibody, Keratin 5 Antibody, KRT5A Antibody, Type-II keratin Kb5 Antibody, Cytokeratin 5 Antibody, K5 Antibody, Keratin-5 Antibody


Specifications

Target
Human KRT5 / CK5 / Cytokeratin 5
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [4A7]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-273 of human KRT5 (NP_000415) produced in E. coli.
Specificity
Human Cytokeratin 5 / Keratin 5
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human bladder tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Human bladder tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-KRT5 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT5.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT5.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human bladder tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Human bladder tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-KRT5 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT5.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT5.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human bladder tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Human bladder tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-KRT5 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT5.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT5.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human bladder tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Human bladder tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-KRT5 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-KRT5 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-KRT5 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT5.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT5.


Requested From: 
Date Requested: 6/23/2018

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