Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 2G3-1C8] (IgG1,k) to Human KRT20 / CK20 / Cytokeratin 20
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Human KRT20 / CK20 / Cytokeratin 20
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [2G3-1C8]
Protein A purified
IHC - Paraffin (10 µg/ml)
Immunofluorescence (10 µg/ml)
Western blot (1:500 - 1:1000)
Specificity and Use
KRT20 / CK20 / Cytokeratin 20 antibody was raised against kRT20 (AAH31559, 1 a.a. ~ 425 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control A-431. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
PBS, pH 7.4.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
KRT20 / CK20 / Cytokeratin 20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa.
Anti-KRT20 / Cytokeratin 20 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4527 concentration 10 ug/ml.
Anti-KRT20 / Cytokeratin 20 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4527 concentration 10 ug/ml.
Immunofluorescence of monoclonal antibody to KRT20 on HeLa cell (antibody concentration 10 ug/ml).
Western blot of KRT20 expression in transfected 293T cell line by KRT20 monoclonal antibody, clone 2G3-1C8.
KRT20 monoclonal antibody, clone 2G3-1C8 Western blot of KRT20 expression in A-431.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged KRT20 is approximately 0.03 ng/ml as a capture antibody.
Requested From: United States
Date Requested: 2/23/2017