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Anti-KRT20 / CK20 / Cytokeratin 20 Antibody (clone 2G3-1C8) IHC-plus™ LS-B4527

Note: This antibody replaces LS-C105150

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular KRT20 / CK20 / Cytokeratin 20 Antibodies

Anti-KRT20 / CK20 / Cytokeratin 20 Antibody (clone IT-Ks 20.10, FITC) IHC-plus™ LS-B5959
Mouse Monoclonal [clone IT-Ks 20.10] (IgG1) to Human KRT20 / CK20 / Cytokeratin 20
Human, Rat, Pig
IHC - Paraffin, Flow Cytometry
FITC Conjugated
Immunohistochemistry Image
Anti-KRT20 / CK20 / Cytokeratin 20 Antibody (Asp409) IHC-plus™ LS-B10488
Rabbit Polyclonal to Human KRT20 / CK20 / Cytokeratin 20
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2G3-1C8] (IgG1,k) to Human KRT20 / CK20 / Cytokeratin 20
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human KRT20 / CK20 / Cytokeratin 20
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [2G3-1C8]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA

Specificity and Use

KRT20 / CK20 / Cytokeratin 20 antibody was raised against kRT20 (AAH31559, 1 a.a. ~ 425 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control A-431. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).

Packaging

PBS, pH 7.4.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About KRT20 / CK20 / Cytokeratin 20

P35900 X73502 CAA51914.1

KRT20 Antibody, Cytokeratin-20 Antibody, Cytokeratin 20 Antibody, CK20 Antibody, K20 Antibody, Keratin 20 Antibody, Keratin-20 Antibody, KRT21 Antibody, CK-20 Antibody, Protein IT Antibody

KRT20 / CK20 / Cytokeratin 20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa.

Immunohistochemistry

Immunohistochemistry
Anti-KRT20 / Cytokeratin 20 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4527 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-KRT20 / Cytokeratin 20 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4527 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to KRT20 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

Western blot
Western blot of KRT20 expression in transfected 293T cell line by KRT20 monoclonal antibody, clone 2G3-1C8.

Western blot

Western blot
KRT20 monoclonal antibody, clone 2G3-1C8 Western blot of KRT20 expression in A-431.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged KRT20 is approximately 0.03 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 12/3/2016

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