Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal to Human KRT20 / CK20 / Cytokeratin 20
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Rabbit Polyclonal (IgG) to Human KRT20 / CK20 / Cytokeratin 20
IHC - Paraffin, ICC, Western blot
Human KRT20 / CK20 / Cytokeratin 20
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:1000)
ICC (1:100 - 1:1000)
Western blot (1:500 - 1:3000)
Specificity and Use
KRT20 / CK20 / Cytokeratin 20 antibody was raised against recombinant fragment corresponding to a region within amino acids 75 and 317 of Cytokeratin 20 (SwissProt P35900). Percent identity by BLAST analysis: Human (100%); Rat (83%); Mouse (82%).
Human Cytokeratin 20 / Keratin 20
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
KRT20 / CK20 / Cytokeratin 20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa.