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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KRT20 / CK20 / Cytokeratin 20 Antibody (aa75‑317) LS‑C186167
Cytokeratin 20 antibody LS-C186167 is an unconjugated rabbit polyclonal antibody to human Cytokeratin 20 (KRT20 / CK20). Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C186167-100
100 µl (1 mg/ml)
Unavailable

Popular KRT20 / CK20 / Cytokeratin 20 Products

Formalin-fixed, paraffin-embedded human colonic carcinoma stained with peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-KRT20 / Cytokeratin 20 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4527 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-KRT20 / Cytokeratin 20 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5959 dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Rat, Pig
Applications: IHC, IHC - Paraffin, Flow Cytometry
Antibody
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
Anti-KRT20 / Cytokeratin 20 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10488 dilution 1:200.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

Cytokeratin 20 antibody LS-C186167 is an unconjugated rabbit polyclonal antibody to human Cytokeratin 20 (KRT20 / CK20). Validated for ICC, IHC and WB.
About KRT20 / CK20 / Cytokeratin 20
KRT20 / CK20 / Cytokeratin 20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. P35900 X73502 CAA51914.1

KRT20 Antibody, Cytokeratin-20 Antibody, Cytokeratin 20 Antibody, CK20 Antibody, K20 Antibody, Keratin 20 Antibody, Keratin-20 Antibody, KRT21 Antibody, CK-20 Antibody, Protein IT Antibody


Specifications

Target
Human KRT20 / CK20 / Cytokeratin 20
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant fragment corresponding to a region within amino acids 75 and 317 of Cytokeratin 20 (SwissProt P35900). Percent identity by BLAST analysis: Human (100%); Rat (83%); Mouse (82%).
Epitope
aa75-317
Specificity
Human Cytokeratin 20 / Keratin 20
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded HT29 xenograft using Cytokeratin 20 antibody at 1:100 dilution.
IHC of paraffin-embedded HT29 xenograft using Cytokeratin 20 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using Cytokeratin 20 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using Cytokeratin 20 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. Cytokeratin 20 / Keratin 20 antibody diluted at 1:3000
Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. Cytokeratin 20 / Keratin 20 antibody diluted at 1:3000

Immunohistochemistry

IHC of paraffin-embedded HT29 xenograft using Cytokeratin 20 antibody at 1:100 dilution.
IHC of paraffin-embedded HT29 xenograft using Cytokeratin 20 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using Cytokeratin 20 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using Cytokeratin 20 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. Cytokeratin 20 / Keratin 20 antibody diluted at 1:3000
Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. Cytokeratin 20 / Keratin 20 antibody diluted at 1:3000

Immunohistochemistry

IHC of paraffin-embedded HT29 xenograft using Cytokeratin 20 antibody at 1:100 dilution.
IHC of paraffin-embedded HT29 xenograft using Cytokeratin 20 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using Cytokeratin 20 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using Cytokeratin 20 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. Cytokeratin 20 / Keratin 20 antibody diluted at 1:3000
Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. Cytokeratin 20 / Keratin 20 antibody diluted at 1:3000

Immunohistochemistry

IHC of paraffin-embedded HT29 xenograft using Cytokeratin 20 antibody at 1:100 dilution.
IHC of paraffin-embedded HT29 xenograft using Cytokeratin 20 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using Cytokeratin 20 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using Cytokeratin 20 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. Cytokeratin 20 / Keratin 20 antibody diluted at 1:3000
Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. Cytokeratin 20 / Keratin 20 antibody diluted at 1:3000


Requested From: 
Date Requested: 6/22/2018

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