Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal to Human KRT2 / CK2 / Cytokeratin 2
Human, Mouse, Rat
IHC, Western blot, Immunoprecipitation, ELISA
Rabbit Polyclonal (IgG) to Human KRT2 / CK2 / Cytokeratin 2
IHC - Paraffin, ICC, Western blot
Human KRT2 / CK2 / Cytokeratin 2
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:1000)
ICC (1:100 - 1:1000)
Western blot (1:500 - 1:3000)
Specificity and Use
KRT2 / CK2 / Cytokeratin 2 antibody was raised against recombinant fragment corresponding to a region within amino acids 143 and 529 of Cytokeratin 2 (SwissProt P35908). Percent identity by BLAST analysis: Human (100%); Dog (82%).
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
KRT2 / CK2 / Cytokeratin 2 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma.