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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KRT2 / CK2 / Cytokeratin 2 Antibody (aa143‑529) LS‑C185501
Cytokeratin 2 antibody LS-C185501 is an unconjugated rabbit polyclonal antibody to human Cytokeratin 2 (KRT2 / CK2). Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C185501-100
100 µl (0.67 mg/ml)
Unavailable

Popular KRT2 / CK2 / Cytokeratin 2 Products

Antibody
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Dog
Applications: IHC, IHC - Paraffin, Western blot
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation

Product Description

Cytokeratin 2 antibody LS-C185501 is an unconjugated rabbit polyclonal antibody to human Cytokeratin 2 (KRT2 / CK2). Validated for ICC, IHC and WB.
About KRT2 / CK2 / Cytokeratin 2
KRT2 / CK2 / Cytokeratin 2 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. P35908 NM_000423 NP_000414.2

KRT2 Antibody, Cytokeratin-2e Antibody, CK-2e Antibody, Epithelial keratin-2e Antibody, K2e Antibody, Keratin 2 Antibody, Keratin-2e Antibody, Krt2e Antibody, KRT2A Antibody, KRTE Antibody, Keratin-2 epidermis Antibody, Type-II keratin Kb2 Antibody


Specifications

Target
Human KRT2 / CK2 / Cytokeratin 2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant fragment corresponding to a region within amino acids 143 and 529 of Cytokeratin 2 (SwissProt P35908). Percent identity by BLAST analysis: Human (100%); Dog (82%).
Epitope
aa143-529
Specificity
Human KRT2
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded H661 xenograft using KRT2 antibody at 1:100 dilution.
IHC of paraffin-embedded H661 xenograft using KRT2 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using Cytokeratin 2 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using Cytokeratin 2 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431
Sample (30 ug of whole cell lysate). A: A431

Immunohistochemistry

IHC of paraffin-embedded H661 xenograft using KRT2 antibody at 1:100 dilution.
IHC of paraffin-embedded H661 xenograft using KRT2 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using Cytokeratin 2 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using Cytokeratin 2 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431
Sample (30 ug of whole cell lysate). A: A431

Immunohistochemistry

IHC of paraffin-embedded H661 xenograft using KRT2 antibody at 1:100 dilution.
IHC of paraffin-embedded H661 xenograft using KRT2 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using Cytokeratin 2 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using Cytokeratin 2 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431
Sample (30 ug of whole cell lysate). A: A431

Immunohistochemistry

IHC of paraffin-embedded H661 xenograft using KRT2 antibody at 1:100 dilution.
IHC of paraffin-embedded H661 xenograft using KRT2 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using Cytokeratin 2 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using Cytokeratin 2 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431
Sample (30 ug of whole cell lysate). A: A431


Requested From: 
Date Requested: 6/19/2018

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