Human KRT18 / CK18 / Cytokeratin 18

Mouse

Human, Mouse, Rat, Bovine, Dog, Hamster, Pig, Sheep (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Ks 18.04

FITC

Protein A purified

Unmodified

Human cytokeratin 18 from HeLa cytoskeletal preparation.

Reacts on cultured lines HeLa and MCf-7. Polypeptide reacting: Mr 45,000 polypeptide of all simple type epithelial and basal cells of many squamous, nonepidermal epithelia.

• IHC
• IHC - Paraffin (1:10)
• IHC - Frozen
• Flow Cytometry

Immunohistochemistry: LS-B3647 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3647 was determined to be 1:10. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

0.09% Sodium Azide

Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05783 NCBI: NM_000224 NP_000215.1