Human KRT18 / CK18 / Cytokeratin 18

Mouse

Human (tested or 100% immunogen sequence identity)

Mouse, Rat, Bovine, Dog, Hamster, Pig, Sheep

IgG1 Monoclonal

B23.1

Unconjugated

Protein G purified

Unmodified. Also available Ready-to-Use, Azide-free, BSA-free.

PMC-42 human breast carcinoma cells were used as the immunogen for the CK18 antibody.

Human KRT18 / CK18 / Cytokeratin 18

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Optimal dilution of the CK18 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05783 NCBI: NM_000224 NP_000215.1