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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KRT18 / CK18 / Cytokeratin 18 Antibody (aa401‑430) IHC‑plus™ LS‑B9323
Note: This antibody replaces LS-C100561
Cytokeratin 18 antibody LS-B9323 is an unconjugated rabbit polyclonal antibody to Cytokeratin 18 (KRT18 / CK18) from human and mouse. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B9323-200
200 µl
Unavailable

Popular KRT18 / CK18 / Cytokeratin 18 Products

Antibody
Species: Human, Mammal
Applications: Flow Cytometry
Anti-KRT18 / Cytokeratin 18 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1669 dilution 1:50.
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen
Anti-KRT18 / CK18 / Cytokeratin 18 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9908 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mammal
Applications: IHC, IHC - Paraffin, Flow Cytometry

Product Description

Cytokeratin 18 antibody LS-B9323 is an unconjugated rabbit polyclonal antibody to Cytokeratin 18 (KRT18 / CK18) from human and mouse. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About KRT18 / CK18 / Cytokeratin 18
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. P05783 NM_000224 NP_000215.1

KRT18 Antibody, CK-18 Antibody, Cytokeratin 18 Antibody, Cytokeratin-18 Antibody, K18 Antibody, Keratin 18 Antibody, CYK18 Antibody, Keratin-18 Antibody


Specifications

Target
Human KRT18 / CK18 / Cytokeratin 18
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8902 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B9323. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa401-430
Specificity
This CYK18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from the C-terminal region of human CYK18.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-KRT18 / CK18 / Cytokeratin 18 antibody IHC staining of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-KRT18 / CK18 / Cytokeratin 18 antibody IHC staining of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Confocal immunofluorescence of CYK18 Antibody with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CYK18 Antibody with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunofluorescence

Confocal immunofluorescence of CYK18 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CYK18 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of CYK18 Antibody in K562,NCI-H460 cell line lysates and mouse stomach tissues lysates(35 ug/lane). CYK18(arrow) was detected using the purified antibody.
Western blot of CYK18 Antibody in K562,NCI-H460 cell line lysates and mouse stomach tissues lysates(35 ug/lane). CYK18(arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of WiDr cells using CYK18 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of WiDr cells using CYK18 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Anti-KRT18 / CK18 / Cytokeratin 18 antibody IHC staining of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-KRT18 / CK18 / Cytokeratin 18 antibody IHC staining of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Confocal immunofluorescence of CYK18 Antibody with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CYK18 Antibody with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunofluorescence

Confocal immunofluorescence of CYK18 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CYK18 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of CYK18 Antibody in K562,NCI-H460 cell line lysates and mouse stomach tissues lysates(35 ug/lane). CYK18(arrow) was detected using the purified antibody.
Western blot of CYK18 Antibody in K562,NCI-H460 cell line lysates and mouse stomach tissues lysates(35 ug/lane). CYK18(arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of WiDr cells using CYK18 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of WiDr cells using CYK18 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Anti-KRT18 / CK18 / Cytokeratin 18 antibody IHC staining of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-KRT18 / CK18 / Cytokeratin 18 antibody IHC staining of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Confocal immunofluorescence of CYK18 Antibody with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CYK18 Antibody with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunofluorescence

Confocal immunofluorescence of CYK18 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CYK18 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of CYK18 Antibody in K562,NCI-H460 cell line lysates and mouse stomach tissues lysates(35 ug/lane). CYK18(arrow) was detected using the purified antibody.
Western blot of CYK18 Antibody in K562,NCI-H460 cell line lysates and mouse stomach tissues lysates(35 ug/lane). CYK18(arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of WiDr cells using CYK18 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of WiDr cells using CYK18 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Anti-KRT18 / CK18 / Cytokeratin 18 antibody IHC staining of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-KRT18 / CK18 / Cytokeratin 18 antibody IHC staining of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Confocal immunofluorescence of CYK18 Antibody with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CYK18 Antibody with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunofluorescence

Confocal immunofluorescence of CYK18 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of CYK18 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of CYK18 Antibody in K562,NCI-H460 cell line lysates and mouse stomach tissues lysates(35 ug/lane). CYK18(arrow) was detected using the purified antibody.
Western blot of CYK18 Antibody in K562,NCI-H460 cell line lysates and mouse stomach tissues lysates(35 ug/lane). CYK18(arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of WiDr cells using CYK18 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of WiDr cells using CYK18 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/25/2018

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