Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat, Human (tested or 100% immunogen sequence identity)
Tissue culture supernatant
IHC - Paraffin
IHC - Frozen
Specificity and Use
Keratin preparation from rat enterocytes.
Recognizes keratin 17 at 46kD. Species cross-reactivity: Human and Rat.
Suitable for use in Immunofluorescence and Immunohistochemistry. Immunohistochemistry (frozen & formalin/paraffin): 1:40-1:80 for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Positive control: Squamous cell carcinoma or skin.
15 mM sodium azide.
Short term: +4°C; Long term: Add glycerol (40-50%) -20°C.
May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.