Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Monoclonal [clone SP95] (IgG) to Human KRT17 / CK17 / Cytokeratin 17
IHC - Paraffin, Western blot, Flow Cytometry
Mouse Monoclonal [clone E3] (IgG2b) to Human KRT17 / CK17 / Cytokeratin 17
IHC - Paraffin, IHC - Frozen, Western blot
Human KRT17 / CK17 / Cytokeratin 17
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [E3]
Tissue culture supernatant
IHC - Paraffin (1:20 - 1:40)
IHC - Frozen (1:20 - 1:40)
Western blot (1:50 - 1:100)
Specificity and Use
Recognizes the 46kD cytokeratin 17, expressed primarily by basal cells of complex epithelia, and with myoepithelial cells in salivary glands and sweat glands. In neoplastic tissues cytokeratin 17 is expressed by squamous cell carcinoma of the lung, cervix and buccal cavity.
Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Citrate buffer pH6.0 is recommended for this purpose.
May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.