Human KRT10 / CK10 / Cytokeratin 10

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

KRT10/1275

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free, Ready-to-Use.

Recombinant human KRT10 protein.

Human KRT10 / CK10 / Cytokeratin 10

• IHC
• IHC - Paraffin (0.1 - 0.2 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

This mAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation. it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts. Optimal dilution of the anti-Keratin 10 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P13645 NCBI: NM_000421 NP_000412.3