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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KRT10 / CK10 / Cytokeratin 10 Antibody (aa146‑195) LS‑C80454
Cytokeratin 10 antibody LS-C80454 is an unconjugated rabbit polyclonal antibody to Cytokeratin 10 (KRT10 / CK10) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C80454-50
50 µg
Unavailable

Popular KRT10 / CK10 / Cytokeratin 10 Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
Formalin-fixed, paraffin-embedded human skin stained with peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epidermal region. This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Rat, Cat, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Immunofluorescence staining of a 7 days old zebrafish embryo
Species: Human, Mouse, Rat, Dog, Pig
Applications: IHC, IHC - Frozen, ICC, Western blot, Flow Cytometry
Anti-KRT10 / Cytokeratin 10 antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6855 dilution 1:200.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-KRT10 / Cytokeratin 10 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7167 concentration 10 ug/ml.
Species: Human, Cat, Dog
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry

Product Description

Cytokeratin 10 antibody LS-C80454 is an unconjugated rabbit polyclonal antibody to Cytokeratin 10 (KRT10 / CK10) from human, mouse, rat and other species. Validated for WB.
About KRT10 / CK10 / Cytokeratin 10
KRT10 / CK10 / Cytokeratin 10 encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. P13645 NM_000421 NP_000412.3

KRT10 Antibody, BIE Antibody, BCIE Antibody, CK10 Antibody, Cytokeratin 10 Antibody, Cytokeratin-10 Antibody, EHK Antibody, Keratin-10 Antibody, KPP Antibody, Keratin 10 Antibody, CK-10 Antibody, K10 Antibody


Specifications

Target
Human KRT10 / CK10 / Cytokeratin 10
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa146-195 of human KRT10 (P13645, NP_000412). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Horse (100%); Opossum, Platypus (85%).
Blocking Peptide
LS-E15355 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80454. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa146-195
Specificity
Human KRT10 / Cytokeratin 10
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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