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Anti-KPNA5 Antibody (clone 1D2) LS-C197583

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular KPNA5 Antibodies

Anti-KPNA5 Antibody (N-Terminus) IHC-plus™ LS-B4728
Rabbit Polyclonal to Human KPNA5
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-KPNA5 Antibody (N-Terminus) IHC-plus™ LS-B9592
Rabbit Polyclonal to Human KPNA5
Human, Mouse
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1D2] (IgG1,k) to Human KPNA5
Human
Western blot, ELISA, RNA interference
Unconjugated

Details

Human KPNA5
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1D2]
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot
  • ELISA
  • RNA interference

Specificity and Use

KPNA5 antibody was raised against kPNA5 (AAH47409.1, aa1-539 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human KPNA5
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About KPNA5

O15131 NM_002269 NP_002260.2

KPNA5 Antibody, Importin subunit alpha-6 Antibody, Karyopherin subunit alpha-5 Antibody, IPOA6 Antibody, Importin alpha 6 Antibody, SRP6 Antibody

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism.

Western blot

Western blot
Western Blot analysis of KPNA5 expression in transfected 293T cell line by KPNA5 monoclonal antibody (M01), clone 1D2.Lane 1: KPNA5 transfected lysate(60.7 KDa).Lane 2: Non-transfected lysate.

Western blot

Western blot
KPNA5 monoclonal antibody (M01), clone 1D2 Western Blot analysis of KPNA5 expression in HepG2.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged KPNA5 is approximately 3 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot analysis of KPNA5 over-expressed 293 cell line, cotransfected with KPNA5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with KPNA5 monoclonal antibody (M01), clone 1D2 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Requested From: United States
Date Requested: 12/7/2016

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