Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
GST fusion protein.
Specific for the NPI-1 (nucleoprotein interactor-1, karyopherin alpha 1, hSRP1) protein. On Western blots, it identifies a band at ~60kD. Reactivity has been confirmed with human HeLa and MDBK cell lysates.
Suitable for use in Immunoprecipitation, Immunofluorescence, ELISA and Western Blot. Immunoprecipitation (native): 5-10 ug. Immunofluorescence: 5-10 ug/ml. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml.
PBS, pH 7.4, 0.1% sodium azide.
Short term: +4°C; Long term: Add glycerol (40-50%) -20°C.
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism.