Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse (tested or 100% immunogen sequence identity)
IHC - Paraffin
Specificity and Use
KMT2A / MLL antibody was raised against a synthetic peptide mapping to a region between residues 720 and 780 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297). The epitope is found in the N-terminal 300 kD fragment generated by proteolytic cleavage. The epitope is found in isoform 14P-18B of MLL1.
Specific for human MLL1 protein. Species cross-reactivity: mouse. Other species have not been tested.
Suitable for use in Western Blot, and Immunohistochemistry (paraffin-embedded sections).
KMT2A Antibody, ALL1 Antibody, CDK6/MLL fusion protein Antibody, FLJ11783 Antibody, HRX Antibody, HTRX Antibody, HTRX1 Antibody, Lysine N-methyltransferase 2A Antibody, MLL1A Antibody, MLL/GAS7 Antibody, MLL1 Antibody, ALL-1 Antibody, MLL Antibody, MLL/GAS7 fusion protein Antibody, TRX1 Antibody, WDSTS Antibody, Trithorax-like protein Antibody, Zinc finger protein HRX Antibody, CXXC7 Antibody, MLL-AF4 der(11) fusion protein Antibody, MLL/GMPS fusion protein Antibody, TET1-MLL Antibody
Histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation.
WB Analysis: Samples: Nuclear extract (50 ug for WT and KO; 10 ug for EF, EN and EC) from 293T cells that were transfected with MLL1 C-terminal 180 kD fragment (EC), 293T cells transfected with N-terminally truncated MLL1 fragment (EN; N300-tr), 293T cells transfected with MLL1 N-terminal 300 kD fragment (EF), MLL1 +/+ mouse embryonic stem cells (WT), and MLL1 -/- embryonic stem cells (KO). Antibody used at the indicated concentrations. Detection: One second exposure with chemiluminescence.
Flow cytometric analysis: 1x10^6 Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml antibody in a 150 ul reaction. Isotype control: black; anti- MLL1: red.
Requested From: United States
Date Requested: 1/21/2017