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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KMT2A / MLL Antibody (aa3084‑3959, clone 4H354) LS‑C16577
MLL antibody LS-C16577 is an unconjugated mouse monoclonal antibody to MLL (KMT2A) from human and mouse. Validated for IP and WB.
Antibody
Catalog
Size
Price
LS-C16577-200
200 µg (1 mg/ml)
$770

Popular KMT2A / MLL Products

WB Analysis: Samples: Nuclear extract (50 ug for WT and KO; 10 ug for EF, EN and EC) from 293T cells that were transfected with MLL1 C-terminal 180 kD fragment (EC), 293T cells transfected with N-terminally truncated MLL1 fragment (EN; N300-tr), 293T cells transfected with MLL1 N-terminal 300 kD fragment (EF), MLL1 +/+ mouse embryonic stem cells (WT), and MLL1 -/- embryonic stem cells (KO). Antibody used at the indicated concentrations. Detection: One second exposure with chemiluminescence.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Detection of Human C-terminal 180 kDa Fragment of MLL1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from K562 (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. The c-terminal 180 kDa Fragment of MLL1 was also immunoprecipitated by a prior lot of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Species: Human, Mouse, Rat, Guinea pig, Horse
Applications: Western blot, Immunoprecipitation, Chromatin Immunoprecipitation, Proximity Ligation Assay
Anti-MLL antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3471 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry

Product Description

MLL antibody LS-C16577 is an unconjugated mouse monoclonal antibody to MLL (KMT2A) from human and mouse. Validated for IP and WB.

Specifications

Target
Human KMT2A / MLL
Synonyms
KMT2A, ALL1, CDK6/MLL fusion protein, FLJ11783, HRX, HTRX, HTRX1, Lysine N-methyltransferase 2A, MLL1A, MLL/GAS7, MLL1, ALL-1, MLL, MLL/GAS7 fusion protein, TRX1, WDSTS, Trithorax-like protein, Zinc finger protein HRX, CXXC7, MLL-AF4 der(11) fusion protein, MLL/GMPS fusion protein, TET1-MLL
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [4H354]
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 1 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
KMT2A / MLL antibody was raised against mBP fusion protein corresponding to residues 3084-3959 of human mixed lineage leukemia (MLL).
Epitope
aa3084-3959
Specificity
Recognizes the C-terminal proteolytic fragment of human MLL (MLLC), Mr ~180kD. Species cross-reactivity: Human and mouse.
Usage
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: Reported by an independent laboratory to immunoprecipitate MLLC from nuclear extracts. The N-terminal fragment, MLLN (300kD), is also co-immunoprecipitated by this antibody.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About KMT2A / MLL
Histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Q03164 NM_005933 NP_005924.2


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Requested From: United States
Date Requested: 10/21/2018

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