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Anti-KMT2A / MLL Antibody (aa3084-3959, clone 4H354) LS-C16577

Catalog Size Price
LS-C16577-200 200 µg (1 mg/ml) Unavailable

Related Products

49 KMT2A / MLL Antibodies

Most Popular KMT2A / MLL Antibodies

Anti-KMT2A / MLL Antibody (aa3084-3959, clone 4H354) LS-C16577
Mouse Monoclonal [clone 4H354] (IgG1) to Human KMT2A / MLL
Human, Mouse
Western blot, Immunoprecipitation
Unconjugated
Anti-KMT2A / MLL Antibody (N-Terminus) LS-C72739
Rabbit Polyclonal to Human KMT2A / MLL
Human, Mouse
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
WB Analysis: Samples: Nuclear extract (50 ug for WT and KO; 10 ug for EF, EN and EC) from 293T cells that were transfected with MLL1 C-terminal 180 kD fragment (EC), 293T cells transfected with N-terminally truncated MLL1 fragment (EN; N300-tr), 293T cells transfected with MLL1 N-terminal 300 kD fragment (EF), MLL1 +/+ mouse embryonic stem cells (WT), and MLL1 -/- embryonic stem cells (KO). Antibody used at the indicated concentrations. Detection: One second exposure with chemiluminescence.
Anti-KMT2A / MLL Antibody (aa3879-3908) LS-C98665
Rabbit Polyclonal to Human KMT2A / MLL
Human
IHC, IHC - Paraffin, Flow Cytometry
Unconjugated
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Anti-KMT2A / MLL Antibody (aa3751-3968, clone 10F8D7) IHC-plus™ LS-B3471
Mouse Monoclonal [clone 10F8D7] (IgG1) to Human KMT2A / MLL
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-MLL antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3471 dilution 1:200.
Anti-KMT2A / MLL Antibody (aa2725-2775) LS-C287274
Rabbit Polyclonal (IgG) to Human KMT2A / MLL
Human, Mouse, Rat, Guinea pig, Horse
Western blot, Immunoprecipitation, Chromatin Immunoprecipitation, Proximity Ligation Assay
Unconjugated
Detection of Human C-terminal 180 kDa Fragment of MLL1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from K562 (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. The c-terminal 180 kDa Fragment of MLL1 was also immunoprecipitated by a prior lot of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

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Mouse Monoclonal [clone 4H354] (IgG1) to Human KMT2A / MLL
Human, Mouse
Western blot, Immunoprecipitation (applications tested for the base form of this product only)
Unconjugated

Details

Human KMT2A / MLL
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [4H354]
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Protein G purified
Unmodified

Applications

  • Western blot (0.1 - 1 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)

Specificity and Use

KMT2A / MLL antibody was raised against mBP fusion protein corresponding to residues 3084-3959 of human mixed lineage leukemia (MLL).
aa3084-3959
Recognizes the C-terminal proteolytic fragment of human MLL (MLLC), Mr ~180kD. Species cross-reactivity: Human and mouse.
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: Reported by an independent laboratory to immunoprecipitate MLLC from nuclear extracts. The N-terminal fragment, MLLN (300kD), is also co-immunoprecipitated by this antibody.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About KMT2A / MLL

Q03164 NM_005933 NP_005924.2

KMT2A Antibody, ALL1 Antibody, CDK6/MLL fusion protein Antibody, FLJ11783 Antibody, HRX Antibody, HTRX Antibody, HTRX1 Antibody, Lysine N-methyltransferase 2A Antibody, MLL1A Antibody, MLL/GAS7 Antibody, MLL1 Antibody, ALL-1 Antibody, MLL Antibody, MLL/GAS7 fusion protein Antibody, TRX1 Antibody, WDSTS Antibody, Trithorax-like protein Antibody, Zinc finger protein HRX Antibody, CXXC7 Antibody, MLL-AF4 der(11) fusion protein Antibody, MLL/GMPS fusion protein Antibody, TET1-MLL Antibody

Histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation.

Requested From: 
Date Requested: 5/20/2018

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