Human KLK9 / Kallikrein 9

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Protein G purified

Unmodified

NS0-derived rhKLK9.

Selected for its ability to detect human KLK9 in direct ELISAs and Western Blots. In Western Blots, this antibody shows no cross-reactivity with rhKLK1, -3, -4, -5, -6, -7, -8, -10, -11, -12, -13, -14, -15, rhKLKB1, rhHGFA, rhFactor VII, rhFactor X, rhFactor XI, or rhThrombin.

• Western blot
• Immunoprecipitation
• ELISA

Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect human KLK9. Using a colorimetric detection system, the detection limit for rhKLK9 is approximately 25 ng/lane and 10 ng/lane under non-reducing and reducing conditions, respectively. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Immunoprecipitation: This antibody was used at a concentration of 25 ug/ml to immunoprecipitate rhKLK9 from conditioned cell culture medium. The recovered rhKLK9 can be detected in Western blots using a polyclonal antibody. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human KLK9. The detection limit for rhKLK9 is approximately 10 ng/well.

Lyophilized from PBS, 5% Trehalose

Reconstitute in 200 ul sterile PBS

Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9UKQ9 NCBI: NM_012315 NP_036447.1