Human KLK13 / Kallikrein 13

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

NS0-derived rhKLK13 (aa19-262).

aa19-262

Selected for its ability to recognize human KLK13 in direct ELISAs and Western Blots. In these formats, this antibody shows ~10% cross-reactivity with rhKallikrein 8, and less than 5% cross-reactivity with rhKallikrein 11, rhKallikrein 14, and rhKallikrein 15.

• Western blot
• Immunoprecipitation
• ELISA
• Neutralization

Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhKLK13 from conditioned media of transfected NS0 cells. The recovered rhKLK13 can be detected by western blot analysis using a monoclonal antibody. Neutralization: This antibody was used to inhibit/neutralize the enzymatic activity of rhKLK13. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate, Boc-VPR-AMC. 50% of the proteolytic activity was inhibited by the antibody at approximately 8.6 ug/ml (IC50) under conditions in which the enzyme was present at 1 ug/ml and the substrate concentration was 100 M. Considering the molecular masses of the enzyme (28 kD) and the antibody (150 kD), IC50 was achieved at approximately 2:1 molar ratio of the antibody to the enzyme. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 1.0 ng/well.

Lyophilized

Reconstitute in deionized water

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9UKR3 NCBI: NM_015596 NP_056411.1