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Anti-KLHL3 Antibody (aa71-120) IHC-plus™ LS-B5609

Note: This antibody replaces LS-C109930
Catalog Size Price
LS-B5609-50 50 µg (1 mg/ml) Unavailable

Related Products

27 KLHL3 Antibodies

Most Popular KLHL3 Antibodies

Anti-KLHL3 Antibody (N-Terminus) LS-C31304
Rabbit Polyclonal (IgG) to Human KLHL3
Human, Bat, Bovine, Guinea pig, Rabbit
Western blot
Unconjugated
Western blot Image
Anti-KLHL3 Antibody (aa71-120) IHC-plus™ LS-B5609
Rabbit Polyclonal to Human KLHL3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-KLHL3 Antibody (aa207-234) LS-C165574
Rabbit Polyclonal to Human KLHL3
Human
Western blot
Unconjugated
Western blot Image
Anti-KLHL3 Antibody (aa14-63) LS-C292124
Rabbit Polyclonal (IgG) to Human KLHL3
Human
IHC, Western blot, ELISA
Unconjugated
Anti-KLHL3 Antibody (aa10-90) LS-C384270
Rabbit Polyclonal (IgG) to Human KLHL3
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated

100% Guaranteed
Rabbit Polyclonal to Human KLHL3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human KLHL3
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Chicken (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

KLHL3 antibody was raised against synthetic peptide located between aa71-120 of human KLHL3 (Q9UH77, NP_059111). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%); Turkey, Zebra finch, Chicken, Lizard (92%); Dog (85%).
LS-E12270 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5609. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa71-120
Human KLHL3
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About KLHL3

Q9UH77 NM_017415 NP_059111.2

KLHL3 Antibody, Kelch-like 3 (Drosophila) Antibody, KIAA1129 Antibody, PHA2D Antibody, Kelch (Drosophila)-like 3 Antibody, Kelch-like protein 3 Antibody

KLHL3 is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex.

Immunohistochemistry

Immunohistochemistry
Anti-KLHL3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5609 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
KLHL3 antibody LS-B5609 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 8/18/2017

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