Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse (tested or 100% immunogen sequence identity)
Rat, Bovine, Monkey-Rhesus (at least 90% immunogen sequence identity)
IHC - Paraffin (10 µg/ml)
Western blot (1:500 - 1:3000)
Specificity and Use
KLHL28 / BTBD5 antibody was raised against recombinant fragment corresponding to a region within amino acids 31 and 278 of KLHL28 (SwissProt Q9NXS3). Percent identity by BLAST analysis: Human (100%); Bovine (99%); Rat (96%).
Human, mouse KLHL28 / BTBD5
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.