LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
KLHL22 Antibody (aa175‑224) LS‑C135849
KLHL22 antibody LS-C135849 is an unconjugated rabbit polyclonal antibody to KLHL22 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C135849-50
50 µg
Unavailable

Popular KLHL22 Products

Immunofluorescence of U251 cells using KLHL22 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: Western blot, ELISA
Western blot. All lanes: KLHL22 antibody at 8 ug/ml. Lane 1: Jurkat whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Secondary antibody: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 72 kDa. Observed band size: 72 kDa.
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

KLHL22 antibody LS-C135849 is an unconjugated rabbit polyclonal antibody to KLHL22 from human, mouse, rat and other species. Validated for WB.
About KLHL22
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation. Q53GT1 NM_032775 NP_116164.2

KLHL22 Antibody, Kelch-like 22 (Drosophila) Antibody, Kelch-like protein 22 Antibody, KELCHL Antibody


Specifications

Target
Human KLHL22
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Guinea pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
KLHL22 antibody was raised against synthetic peptide located between aa175-224 of human KLHL22 (Q53GT1, NP_116164). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig (100%); Opossum, Guinea pig (92%).
Blocking Peptide
LS-E22195 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135849. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa175-224
Specificity
Human KLHL22
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Western blot

KLHL22 antibody LS-C135849 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
KLHL22 antibody LS-C135849 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

KLHL22 antibody LS-C135849 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
KLHL22 antibody LS-C135849 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

KLHL22 antibody LS-C135849 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
KLHL22 antibody LS-C135849 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

KLHL22 antibody LS-C135849 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
KLHL22 antibody LS-C135849 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number