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Anti-KLHL20 Antibody (aa481-530) LS-C117149

Catalog Size Price
LS-C117149-50 50 µg Unavailable

Related Products

8 KLHL20 Antibodies

Most Popular KLHL20 Antibodies

Anti-KLHL20 Antibody (aa481-530) LS-C117149
Rabbit Polyclonal to Human KLHL20
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Insect
Western blot
Unconjugated
KLHL20 antibody LS-C117149 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-KLHL20 Antibody LS-C680736
Rabbit Polyclonal (IgG) to Human KLHL20
Human
IHC, Immunofluorescence, ELISA
Unconjugated
Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Anti-KLHL20 Antibody LS-C680739
Rabbit Polyclonal (IgG) to Human KLHL20
Human
ELISA
Unconjugated
Anti-KLHL20 Antibody LS-C680741
Rabbit Polyclonal (IgG) to Human KLHL20
Human
ELISA
Unconjugated
Anti-KLHL20 Antibody LS-C680742
Rabbit Polyclonal (IgG) to Human KLHL20
Human
ELISA
Unconjugated

100% Guaranteed
Rabbit Polyclonal to Human KLHL20
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Insect
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human KLHL20
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Insect (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

KLHL20 antibody was raised against synthetic peptide located between aa481-530 of human KLHL20 (Q9Y2M5, NP_055273). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus, Stickleback, Zebrafish, Mosquito (100%); Drosophila, Beetle (85%).
LS-E19876 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C117149. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa481-530
Human KLHL20
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

About KLHL20

Q9Y2M5 NM_014458 NP_055273.2

KLHL20 Antibody, Kelch-like protein 20 Antibody, KLEIP Antibody, KLHLX Antibody, Kelch-like 20 (Drosophila) Antibody, KHLHX Antibody, RP3-383J4.3 Antibody, Kelch motif containing protein Antibody, Kelch-like protein X Antibody

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis (PubMed:20389280). The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'-linked ubiquitination (PubMed:24768539).

Western blot

KLHL20 antibody LS-C117149 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
KLHL20 antibody LS-C117149 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/22/2018

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