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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KLHDC8B Antibody (aa259‑270) LS‑C154804
KLHDC8B antibody LS-C154804 is an unconjugated goat polyclonal antibody to KLHDC8B from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C154804-100
100 µg (0.5 mg/ml)
Unavailable

Popular KLHDC8B Products

Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

KLHDC8B antibody LS-C154804 is an unconjugated goat polyclonal antibody to KLHDC8B from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
About KLHDC8B
KLHDC8B encodes a protein which forms a distinct beta-propeller protein structure of kelch domains allowing for protein-protein interactions. Mutations in this gene have been associated with Hodgkin lymphoma. Q8IXV7 Q8IXV7

KLHDC8B Antibody, Kelch domain containing 8B Antibody


Specifications

Target
Human KLHDC8B
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Guinea pig, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Immunogen
KLHDC8B antibody was raised against synthetic peptide C-TKLPRSLRMRDK from an internal region of human KLHDC8B (NP_775817.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Hamster, Panda, Rabbit (100%); Elephant, Horse, Porcine, Opossum, Guinea pig (92%).
Blocking Peptide
LS-E27545 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C154804. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa259-270
Specificity
Human KLHDC8B.
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 38kD band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 37.7kD according to NP_775817.1). Recommended concentration: 0.5-2 ug/ml. An additional band of unknown identity was also consistently observed at 22kD. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

KLHDC8B antibody (0.5 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
KLHDC8B antibody (0.5 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

KLHDC8B antibody (0.5 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
KLHDC8B antibody (0.5 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

KLHDC8B antibody (0.5 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
KLHDC8B antibody (0.5 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

KLHDC8B antibody (0.5 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
KLHDC8B antibody (0.5 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/21/2018

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