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KLH

KLH / Keyhole Limpet Hemocyanin

Keyhole limpet hemocyanin (KLH) is a large, multisubunit, oxygen-carrying, metalloprotein that is found in the hemolymph of the giant keyhole limpet, Megathura crenulata, a species of keyhole limpet that lives off the coast of California, from Monterey Bay to Isla Asuncion off Baja California. KLH is used extensively as a carrier protein in the production of antibodies for research, biotechnology and therapeutic applications. Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production. KLH is the most widely employed carrier protein for this purpose. KLH is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens allows a high hapten:carrier protein ratio, increasing the likelihood of generating hapten-specific antibodies. In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically-based research techniques in mammalian model organisms.

KLH Target Details

Target Name: KLH / Keyhole Limpet Hemocyanin
Target Abbreviation: KLH

☰ Filters
Antibodies (42)
Primary (42)
KLH (42)
Human (1)
Keyhole Limpet (32)
Megathura crenulata (1)
IHC (8)
WB (19)
Flo (1)
ELISA (28)
FLISA (3)
ICC (1)
IF (4)
rabbit (13)
mouse (20)
chicken (9)
IgG (9)
IgG1 (9)
IgG1,k (3)
IgG2a (1)
IgG3 (1)
IgM (4)
IgY (4)
monoclonal mc (20)
polyclonal pc (22)
11E8F5C3 (2)
12B4.G3.A8 (1)
3B1 (2)
6D12 (1)
Clone 1 (1)
KT82 (1)
KT83 (1)
KT85 (1)
Agarose Conjugated (1)
AP Conjugated (1)
APC Conjugated (1)
Biotin Conjugated (7)
FITC Conjugated (5)
HRP Conjugated (4)
PE Conjugated (1)
Preservative Free (2)
TR Conjugated (1)
TRITC Conjugated (1)
Unconjugated (20)
No (42)
Select
KLH Mouse anti-Keyhole Limpet Monoclonal (12B4.G3.A8) Antibody
Keyhole Limpet
ELISA, IHC, WB
Unconjugated
500 µg/$435
KLH Antibody - Anti-KLH Polyclonal Antibody - Western blot. rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody was used to detect KLH under reducing conditions. Membrane was Blocked in 1% BSA-TTBS for 30 min RT and incubated with rabbit anti-KLH, 1:1000 in 1% BSA-TBS, overnight at 4°C. Primary antibody was detected with HRP Goat-anti-Rabbit LS-C60865 Lot#21231 (1:40000 in MB-070 30 min RT) and imaged on the BioRad Versa Doc imaging system. Other detection systems will yield similar results.
Select
KLH Rabbit anti-Keyhole Limpet Polyclonal Antibody
Keyhole Limpet
ELISA, IHC, WB
Unconjugated
1 mg/$738
Select
KLH Rabbit anti-Keyhole Limpet Polyclonal (FITC) Antibody
Keyhole Limpet
Flo, IF, WB
FITC Conjugated
100 µl/$884
Select
KLH Mouse anti-Keyhole Limpet Monoclonal Antibody
Keyhole Limpet
ELISA
Unconjugated
1 mg/$538
Select
KLH Mouse anti-Keyhole Limpet Monoclonal (3B1) Antibody
Keyhole Limpet
ELISA, IHC, WB
Unconjugated
100 µg/$574
Select
KLH Chicken anti-Keyhole Limpet Polyclonal (HRP) Antibody
Keyhole Limpet
ELISA
HRP Conjugated
500 µg/$513
Select
KLH Rabbit anti-Keyhole Limpet Polyclonal (Biotin) Antibody
Keyhole Limpet
ELISA, IHC, WB
Biotin Conjugated
100 µg/$884
KLH Antibody - Anti-KLH Polyclonal Antibody - Western blot. rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody was used to detect KLH under reducing conditions. Membrane was Blocked in 1% BSA-TTBS for 30 min RT and incubated with rabbit anti-KLH, 1:1000 in 1% BSA-TBS, overnight at 4°C. Primary antibody was detected with HRP Goat-anti-Rabbit LS-C60865 Lot#21231 (1:40000 in MB-070 30 min RT) and imaged on the BioRad Versa Doc imaging system. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
KLH Rabbit anti-Keyhole Limpet Polyclonal (Biotin) Antibody
Keyhole Limpet
ELISA, IHC, WB
Biotin Conjugated
100 µg/$544
Select
KLH Chicken anti-Keyhole Limpet Polyclonal Antibody
Keyhole Limpet
ELISA
Unconjugated
500 µg/$513
Select
KLH Mouse anti-Keyhole Limpet Monoclonal (Clone 1) Antibody
Keyhole Limpet
Unconjugated
1 mg/$291
Select
KLH Mouse anti-Keyhole Limpet Monoclonal (KT83) Antibody
Keyhole Limpet
Unconjugated
100 µg/$437
Select
KLH Rabbit Polyclonal Antibody
ELISA, ICC, IHC, WB
Unconjugated
1 ml/$281
Select
KLH Mouse anti-Keyhole Limpet Monoclonal (3B1) Antibody
Keyhole Limpet
ELISA, IHC, WB
Unconjugated
25 µl/$304
KLH Antibody - Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody. Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT.
Select
KLH Rabbit anti-Keyhole Limpet Polyclonal (HRP) Antibody
Keyhole Limpet
ELISA, IHC, WB
HRP Conjugated
100 µg/$544
Select
KLH Mouse anti-Megathura crenulata Monoclonal (6D12) Antibody
Megathura crenulata
ELISA, WB
Unconjugated
50 µg/$440; 100 µg/$468
Select
KLH Mouse anti-Keyhole Limpet Monoclonal (Preservative Free) (11E8F5C3) Antibody
Keyhole Limpet
ELISA, WB
Unconjugated, Preservative Free
20 µg/$242; 100 µg/$304; 200 µg/$334; 500 µg/$452
Select
KLH Mouse anti-Keyhole Limpet Monoclonal (Preservative Free) (Biotin) (11E8F5C3) Antibody
Keyhole Limpet
ELISA, WB
Biotin Conjugated, Preservative Free
50 µg/$343
Select
KLH Mouse anti-Keyhole Limpet Monoclonal Antibody
Keyhole Limpet
ELISA, WB
Unconjugated
20 µl/$271; 100 µl/$397
KLH Antibody - Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody. Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT.
Select
KLH Rabbit anti-Keyhole Limpet Polyclonal (TRITC) Antibody
Keyhole Limpet
FLISA, IF, WB
TRITC Conjugated
100 µl/$544
KLH Antibody - Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody. Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT.
Select
KLH Rabbit anti-Keyhole Limpet Polyclonal (FITC) Antibody
Keyhole Limpet
FLISA, IF, WB
FITC Conjugated
100 µl/$544
KLH Antibody - Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody. Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT.
Select
KLH Rabbit anti-Keyhole Limpet Polyclonal (TR) Antibody
Keyhole Limpet
FLISA, IF, WB
TR Conjugated
100 µl/$544
Select
KLH Rabbit Polyclonal Antibody
ELISA, WB
Unconjugated
1 Each/$431
Select
KLH Rabbit Polyclonal (Biotin) Antibody
ELISA, WB
Biotin Conjugated
1 ml/$307
KLH Antibody - Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody. Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT.
Select
KLH Rabbit anti-Keyhole Limpet Polyclonal (AP) Antibody
Keyhole Limpet
ELISA, WB
AP Conjugated
100 µg/$544
Select
KLH Mouse anti-Keyhole Limpet Monoclonal Antibody
Keyhole Limpet
ELISA
Unconjugated
1 mg/$451
Viewing 1-25 of 42 product results



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).