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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KLF8 Antibody (aa74‑123) LS‑C82007
KLF8 antibody LS-C82007 is an unconjugated rabbit polyclonal antibody to KLF8 from human, mouse and guinea pig. Validated for WB.
Catalog
Size
Price
LS-C82007-50
50 µg
Unavailable

Popular KLF8 Products

KLF8 antibody LS-C29760 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Bovine, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Anti-KLF8 antibody IHC staining of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
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Species: Human
Applications: IHC, Peptide Enzyme-Linked Immunosorbent Assay
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Species: Human
Applications: Western blot, ELISA
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Applications: IHC, ELISA

Product Description

KLF8 antibody LS-C82007 is an unconjugated rabbit polyclonal antibody to KLF8 from human, mouse and guinea pig. Validated for WB.
About KLF8
KLF8 is a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants. O95600 NM_007250 NP_009181.2

KLF8 Antibody, Basic kruppel-like factor 3 Antibody, BKLF3 Antibody, DXS741 Antibody, Krueppel-like factor 8 Antibody, Kruppel-like factor 8 Antibody, Zinc finger protein 741 Antibody, Basic krueppel-like factor 3 Antibody, ZNF741 Antibody


Specifications

Target
Human KLF8
Host
Rabbit
Reactivity
Human, Mouse, Guinea pig (tested or 100% immunogen sequence identity)
Predicted
Pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
KLF8 antibody was raised against synthetic peptide located between aa74-123 of human KLF8 (O95600, NP_009181). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%); Rabbit (92%); Pig (90%); Monkey, Bat, Guinea pig (85%); Mouse (78%).
Blocking Peptide
LS-E21194 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C82007. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa74-123
Specificity
Human KLF8
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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